PMC

GO analysis of DE RNAs. The GO enrichment analysis of DE RNAs is represented by the pie charts. GO, gene ontology; DE, differentially expressed.

Putative targets for Xuefu Zhuyu decoction, a treatment for Qi stagnation and blood stasis syndrome. The triangular nodes represent the screened ingredients and the circular nodes represent the predicted targets.

Network of the targets of Xuefu Zhuyu decoction (treatment for QSBSS) and differentially expressed RNAs in QSBSS. The green triangular nodes represent active components, the green circular nodes represent targets and the red nodes represent shared targets of QSBSS and Xuefu Zhuyu Decoction. QSBSS, Qi stagnation and blood stasis syndrome.

Analysis of DE RNAs by ClueGo. The enrichment analysis of DE RNAs was performed using ClueGo and the most significant terms are presented, where a higher score indicates a higher number of genes involved. The same color indicates that it was possible to cluster the respective terms. DE, differentially expressed.

Enriched Kyoto Encyclopedia of Genes and Genomes pathways. The pathway scatterplot displays the statistics of the pathway enrichment of the differentially expressed RNAs. MAPK, mitogen-activated protein kinase; NF, nuclear factor; Fox, forkhead box; AMPK, 5′AMP-activated protein kinase; NOD, nucleotide-binding oligomerization domain.

Venn diagram of overlapped DE lncRNAs, circRNAs and mRNAs. Venn diagrams displaying the number of overlapped up-regulated (upper panel) and down-regulated (lower panel) DE (A) lncRNAs, (B) circRNAs and (C) mRNAs among groups a, b and c. Groups: (a) QSBSS patients with coronary heart disease (n=5); (b) QSBSS patients with chronic gastritis (n=5); (c) QSBSS patients with rheumatoid arthritis (n=5). QSBSS, Qi stagnation and blood stasis syndrome; lncRNA, long non-coding RNA; circRNA, circular RNA; DE, differentially expressed.

Volcano plot and clustering map of DE mRNAs. Upper panel, volcano plots indicating up- and down-regulated mRNAs for the comparison of the normal controls with groups (A) a, (B) b and (C) c. The respective heat maps presenting the hierarchical clustering of DE mRNAs are displayed in the lower panel. Groups: a, QSBSS patients with coronary heart disease (n=5); b, QSBSS patients with chronic gastritis (n=5); c, QSBSS patients with rheumatoid arthritis (n=5). QSBSS, Qi stagnation and blood stasis syndrome; DE, differentially expressed.

Volcano plot and clustering map of DE circRNAs. Upper panel, volcano plots indicating up- and down-regulated circRNAs for the comparison of the normal controls with groups (A) a, (B) b and (C) c. The respective heat maps presenting the hierarchical clustering of DE circRNAs are displayed in the lower panel. Groups: a, QSBSS patients with coronary heart disease (n=5); b, QSBSS patients with chronic gastritis (n=5); c, QSBSS patients with rheumatoid arthritis (n=5). QSBSS, Qi stagnation and blood stasis syndrome; circRNA, circular RNA; DE, differentially expressed.

Volcano plot and clustering map of DE lncRNAs. Upper panel, volcano plots indicating up- and down-regulated lncRNAs for the comparison of the normal controls with groups (A) a, (B) b and (C) c. The respective heat maps presenting the hierarchical clustering of DE lncRNAs are displayed in the lower panel. Groups: a, QSBSS patients with coronary heart disease (n=5); b, QSBSS patients with chronic gastritis (n=5); c, QSBSS patients with rheumatoid arthritis (n=5). QSBSS, Qi stagnation and blood stasis syndrome; lncRNA, long non-coding RNA; DE, differentially expressed.