Platform: Sequenom Forward primer: ACGTTGGATGATGGTGGCTGTAGGAATCTG, Concentration: 200 nM Reverse primer: ACGTTGGATGGCCTCATGGCCTAAAATCAC, Concentration: 200 nM Extension probe: GGTTAGTGGCAACCTGGTG, Mass: 5899.8, Concentration: 600 nM, Terminator: CGT, Strand: + Extension products: Extension product 1: GGTTAGTGGCAACCTGGTGT, Mass: 6188, Allele: T Extension product 2: GGTTAGTGGCAACCTGGTGAC, Mass: 6486, Allele: A Pause: GGTTAGTGGCAACCTGGTGA, Mass: 6213.1
Platform: Sequencing Primer F: TGAATAACCTTGGGGATAAAC, Temp: 47.9, GC Content:38.1, Product Size: 397 Primer R: CACTTTCGTCTCTCATTGC, Temp: 45.1, GC Content:47.37
Platform: TaqMan Forward primer: ACCTGGTGACCCC, Amount: 900 nM Reverse primer: ACCTGGTGTCCCC, Amount: 900 nM Probe 1: CTCATGGCCTAAAATCACAGGAA, Amount: 150 nM Probe 2: TCTGTAGATGGTGGCTGTAGGAATC, Amount: 300 nM Annealing temperature: 58 C
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