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dbSNP

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Method Detail
Submitter Method Handle: ABI
Submitter Method ID: TAQMAN_GENOTYPING_ASSAY
Submitted method description:
Genotyping was performed with commercially available TaqManŽ Validated SNP Genotyping Assays from Applied Biosystems (Foster City, CA, USA), following the standard protocol suggested by the manufacturer utilizing 3ng of dried down DNA. A high-throughput
assay design pipeline used in the TaqManŽ Custom SNP Genoptyping Assay design service (Applied Biosystems, Foster City, CA) was used to develop 5' nuclease allelic discrimination assays. After the design of primers and TaqManŽ probes, a computational qual
ity-control step was performed to ensure uniqueness of the predicted amplicons in the genome assembly. This eliminated potentially problematic SNP targets that may arise from repeated genomic regions, pseudo-SNPs, and other possible assembly artifacts. Th
e average genotyping error rate in our production lab was estimated at about 0.1% by routinely running duplicate control plates.

This method was used in the following submission:

Submitter Handle Batch Type Submitter batch id Release build id
ABI Frequency xplore_hum_chrY_1 125
ABI Frequency xplore_hum_chr10_1 125
ABI Frequency xplore_hum_chr11_1 125
ABI Frequency xplore_hum_chr12_1 125
ABI Frequency xplore_hum_chr13_1 125
ABI Frequency xplore_hum_chr14_1 125
ABI Frequency xplore_hum_chr15_1 125
ABI Frequency xplore_hum_chr16_1 125
ABI Frequency xplore_hum_chr17_1 125
ABI Frequency xplore_hum_chr18_1 125
ABI Frequency xplore_hum_chr19_1 125
ABI Frequency xplore_hum_chr1_1 125
ABI Frequency xplore_hum_chr20_1 125
ABI Frequency xplore_hum_chr21_1 125
ABI Frequency xplore_hum_chr22_1 125
ABI Frequency xplore_hum_chr2_1 125
ABI Frequency xplore_hum_chr3_1 125
ABI Frequency xplore_hum_chr4_1 125
ABI Frequency xplore_hum_chr5_1 125
ABI Frequency xplore_hum_chr6_1 125
ABI Frequency xplore_hum_chr7_1 125
ABI Frequency xplore_hum_chr8_1 125
ABI Frequency xplore_hum_chr9_1 125
ABI Frequency xplore_hum_chrU_1 125
ABI Frequency xplore_hum_chrX_1 125