| Chromosome 20 specific sequencing libaries were created from the flow sorted Coriell DNA NA10470 (Pygmy origin). |
| We aligned the sequencing reads to build33 of the human genome, available at ftp://ftp.ncbi.nih.gov/genomes/H_sapiens/, using ssahaSNP. The settings for ssahaSNP were such that the candidate SNP base in the read was at least Phred Q=23, that its neighbour |
| ing 5 bases on either side of the candidate SNP all had Phred quality values of >=15 and at least 9 of the 10 neighbours match. If the number of detected SNPs for the aligned segment of a read exceeded a rate of 15 SNPs per 1000 bases then that read align |
| ment was ignored. If a read aligned to more than one place in the genome, then only the longest alignment with the fewest SNPs was reported. |
| Note: Flow sorting of chromosomes also includes a small percentage of unintended chromosomal material. Therefore some reads for this flow sorted library may generate SNPs on other chromosomes. |