| | We aligned 124,520 human reads, available at http://www.ncbi.nih.gov/Traces, to build34 of the human genome, available at ftp://ftp.ncbi.nih.gov/genomes/H_sapiens/, using ssahaSNP. The settings for ssahaSNP were such that the candidate SNP base in the rea |
| | d was at least Phred Q=23, that its neighbouring 5 bases on either side of the candidate SNP all had Phred quality values of >=15 and at least 9 of the 10 neighbours match. If the number of detected SNPs for the aligned segment of a read exceeded a rate o |
| | f 15 SNPs per 1000 bases then that read alignment was ignored. If a read aligned to more than one place in the genome, then only the longest alignment with the fewest SNPs was reported. |
| | Chromosome 16 specific sequencing libaries were created from the flow sorted Coriell DNA NA17109 (African-American origin) at the Wellcome Trust Sanger Institute. Trace data was generated by Human Genome Sequencing Center, Baylor College of Medicine thro |
| | ugh a grant from NIH. This SNP analysis was performed with permission from BCM. |
| | Note: Flow sorting of chromosomes also includes a small percentage of unintended chromosomal material. Therefore some reads for this flow sorted library may generate SNPs on other chromosomes. |
