| | Individual chromosomes were separated using a rodent-human somatic |
| | cell hybrid technique. Clones with unique copies of the target |
| | chromosome were selected using data from hybridization on Affymetrix |
| | HuSNP arrays. Target DNA was prepared by long range PCR of fragments |
| | of 3-14kb. Pooled PCR products were then hybridized to high density |
| | oligonucleotide arrays designed to interrogate genotypes at all |
| | non-repetitive sequence positions covered by these fragments. Wafers |
| | were stained with streptavidin R-phycoerythrin and scanned using |
| | custom built confocal scanners. Polymorphic sites were identified by |
| | recognition of altered hybridization patterns. |
