Callinectin, an Antibacterial Peptide from Blue Crab, Callinectes sapidus, Hemocytes

L Khoo; DW Robinette; EJ Noga

doi:10.1007/pl00011750

Callinectin, an Antibacterial Peptide from Blue Crab, Callinectes sapidus, Hemocytes

Mar Biotechnol (NY). 1999 Jan;1(1):44-51. doi: 10.1007/pl00011750.

Authors

L Khoo¹, DW Robinette, EJ Noga

Affiliation

¹ North Carolina State University, College of Veterinary Medicine, 4700 Hillsborough Street, Raleigh, NC 27606, USA

PMID: 10373609
DOI: 10.1007/pl00011750

Abstract

This paper describes the isolation of an approximately 3.7 kDa, basic, antibacterial peptide (designated callinectin), which represents the major antibiotic activity in blue crab, Callinectes sapidus, hemocytes. A single-step purification using low-pressure cation-exchange chromatology yielded a highly purified (>95%) peptide. Purity was confirmed by C4 reverse-phase high-performance liquid chromatography (RP-HPLC), native gel electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), capillary electophoresis, and mass spectral analysis. The partial amino acid sequence obtained via Edman degradation revealed no significant homology to other reported peptides in the Basic Local Alignment Search Tool (BLAST) program database.