Alfalfa (Medicago sativa L.) is of immense importance as livestock fodder for both developed and lesser developed countries as it contains a high amount of protein, and yield of dry matter is also very high. The main antinutritional components present in this plant are saponins, and their unfavorable effects on animal performance have restricted the optimum use of this high-protein plant as an animal feed. The occurrence, chemistry, analysis, and consequences of intake of alfalfa saponins are reviewed. The information synthesized may lead to planning of more detailed studies on isolation and characterization of saponins and sapogenins to gain a better understanding of the biological activities of the aglycon and carbohydrate moieties of saponins.