The nucleotide sequence and genome organization of Sclerophthora macrospora virus B

Virology. 1999 Nov 25;264(2):344-9. doi: 10.1006/viro.1999.0018.

Abstract

Sclerophthora macrospora Virus B (SmV B) found in S. macrospora, the pathogenic fungus responsible for downy mildew in gramineous plants, is a small icosahedral, monopartite virus containing a positive-strand ssRNA genome. In the present study, the complete nucleotide sequence of the SmV B genome was determined. The viral genome consists of 5533 nucleotides and has two large open reading frames (ORFs). ORF1 encodes a putative polyprotein containing the motifs of chymotrypsin-related serine protease and RNA-directed RNA polymerase. ORF2 encodes a capsid protein. The deduced amino acid sequence shows some similarity to those of certain positive-strand RNA viruses, but the genome organization is characteristic and distinct from those of other known fungal RNA viruses. These results suggest that SmV B should be classified into a new group of mycoviruses.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Capsid / genetics
  • DNA, Viral / analysis*
  • Genome, Viral*
  • Molecular Sequence Data
  • Oomycetes / virology
  • Open Reading Frames
  • RNA Viruses / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Viral

Associated data

  • GENBANK/AB012756