Abstract
Phosphorylation of anti-viral nucleoside analogs by mitochondrial thymidine kinase 2 (TK2) has been implicated as a mechanism for the mitochondrial toxicity caused by several of these compounds. We have cloned the cDNA of mouse TK2 and shown that the enzyme is targeted to the mitochondria when expressed in fusion with the green fluorescent protein. This is the first report on the cloning of a mitochondrial TK2 and will contribute to elucidate the role of TK2 in the pharmacological activation of nucleoside analogs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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CHO Cells
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Cloning, Molecular
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Cricetinae
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Green Fluorescent Proteins
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Luminescent Proteins
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Mice
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Mitochondria / enzymology*
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Molecular Sequence Data
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Nucleosides / metabolism
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Phosphorylation
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Plasmids
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Recombinant Fusion Proteins / chemistry
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Sequence Alignment
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Thymidine Kinase / chemistry
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Thymidine Kinase / genetics*
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Transfection
Substances
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Luminescent Proteins
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Nucleosides
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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thymidine kinase 2
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Thymidine Kinase