Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily

J Biol Chem. 2000 Jan 14;275(2):861-6. doi: 10.1074/jbc.275.2.861.

Abstract

We describe the characterization of siglec-8, a novel sialic acid-binding immunoglobulin-like lectin that is expressed specifically by eosinophils. A full-length cDNA encoding siglec-8 was isolated from a human eosinophil cDNA library. Siglec-8 is predicted to contain three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail of 47 amino acids. The siglec-8 gene mapped on chromosome 19q13.33-41, closely linked to genes encoding CD33 (siglec-3), siglec-5, siglec-6, and siglec-7. When siglec-8 was expressed on COS cells or as a recombinant protein fused to the Fc region of human IgG(1), it was able to mediate sialic acid-dependent binding to human erythrocytes and to soluble sialoglycoconjugates. Using specific monoclonal antibodies, siglec-8 could be detected only on eosinophils and hence appears to be the first example of an eosinophil-specific transmembrane receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / chemistry*
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism
  • Antigens, Differentiation, B-Lymphocyte / chemistry*
  • Antigens, Differentiation, B-Lymphocyte / genetics*
  • Antigens, Differentiation, B-Lymphocyte / metabolism
  • Antigens, Differentiation, Myelomonocytic / chemistry
  • Antigens, Differentiation, Myelomonocytic / genetics
  • COS Cells
  • Chromosome Mapping
  • Chromosomes, Human, Pair 19*
  • Eosinophils / immunology*
  • Erythrocytes / immunology
  • Gene Library
  • Genetic Linkage
  • Granulocytes / immunology
  • Humans
  • Immunoglobulin Fc Fragments / genetics
  • Immunoglobulin G / genetics
  • Lectins*
  • Lymphocytes / immunology
  • Molecular Sequence Data
  • Monocytes / immunology
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sialic Acid Binding Ig-like Lectin 3
  • Sialic Acids / blood
  • Transfection

Substances

  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, Myelomonocytic
  • CD33 protein, human
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Lectins
  • Recombinant Fusion Proteins
  • SIGLEC7 protein, human
  • SIGLEC8 protein, human
  • Sialic Acid Binding Ig-like Lectin 3
  • Sialic Acids