Abstract
The full length of a gene isolated using a methylated DNA binding column as previously reported (Cross et al., 1994) has been cloned. The gene encodes 55 amino acids (6.2 kDa), and has a transcript of approximately 0.45 kb. It has 2 exons in the genome. After searching for sequence homology (BLAST), a homology to hypothetically 6.3 kDa protein located at cosmid ZK652 (protein P34660; EMBL Accession No. L14429) of C. elegans chromosome 3 was noted (51.9% amino acid homology). It has 1 site (-SQ-) for DNA-dependent protein kinase (DNA-PK) phosphorylation and 2 sites of myristilation. This novel gene is very strongly expressed (about 25.6 times) in DNA-PK-deleted human glioma cell line (M059J) 2 hrs after ultraviolet (UV) irradiation compared to the DNA-PK +/+ cell line (M059K) which expresses the gene at a relatively low level (about 7.5 times). Therefore, this result suggests that DNA-PK may negatively regulate the transcriptional level of this gene in M059K cells upon UV damage (50 J/m2), associated with cell toxicity.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Survival / radiation effects
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Central Nervous System Neoplasms / genetics*
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Central Nervous System Neoplasms / radiotherapy*
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Cloning, Molecular
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DNA Damage / radiation effects
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DNA Methylation
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DNA-Activated Protein Kinase
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DNA-Binding Proteins*
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Dose-Response Relationship, Radiation
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Gene Expression Regulation, Neoplastic / radiation effects
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Glioma / enzymology
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Glioma / genetics*
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Glioma / radiotherapy*
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Helminth Proteins / genetics
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Helminth Proteins / metabolism
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Humans
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Membrane Proteins
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Mitochondrial Precursor Protein Import Complex Proteins
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Mitochondrial Proteins
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Molecular Sequence Data
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Nuclear Proteins
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Phosphorylation
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism
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Protein Serine-Threonine Kinases / radiation effects
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Proteins / genetics*
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Proteins / metabolism*
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
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Ultraviolet Rays
Substances
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DNA-Binding Proteins
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Helminth Proteins
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Membrane Proteins
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Mitochondrial Precursor Protein Import Complex Proteins
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Mitochondrial Proteins
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Nuclear Proteins
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Proteins
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TOMM7 protein, human
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DNA-Activated Protein Kinase
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PRKDC protein, human
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Protein Serine-Threonine Kinases