A putative 12 transmembrane domain cotransporter expressed in thymic cortical epithelial cells

J Immunol. 2000 Mar 15;164(6):3185-92. doi: 10.4049/jimmunol.164.6.3185.

Abstract

We have isolated a full-length cDNA clone (thymic stromal origin (TSO)-1C12) from a SCID thymus library using a probe from a PCR-based subtractive library enriched for sequences from fetal thymic stromal cells. TSO-1C12 mRNA is expressed mainly in the thymic cortex and is highly enriched in SCID thymus. Expression per cell is highest during fetal thymus development and decreases after day 16. Antipeptide Abs immunoprecipitated a hydrophobic, plasma membrane glycoprotein (thymic stromal cotransporter, TSCOT) whose translated sequence has weak homology to bacterial antiporters and mammalian cation cotransporters with 12 transmembrane domains. TSCOT represents a new member of this superfamily that is highly expressed in thymic cortical epithelial cells.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Epithelial Cells / metabolism*
  • Epithelial Cells / physiology
  • Female
  • Gene Expression Regulation, Developmental / immunology
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, SCID
  • Molecular Sequence Data
  • Protein Structure, Tertiary / genetics
  • Stromal Cells / metabolism
  • Stromal Cells / physiology
  • Symporters*
  • Thymus Gland / cytology
  • Thymus Gland / growth & development
  • Thymus Gland / immunology
  • Thymus Gland / metabolism*

Substances

  • Carrier Proteins
  • DNA, Complementary
  • Membrane Glycoproteins
  • Symporters
  • Tscot protein, mouse

Associated data

  • GENBANK/AF148145