Abstract
Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Amino Acid Sequence
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Animals
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Autophagy-Related Protein 8 Family
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Base Sequence
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Biological Transport, Active
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Brain / metabolism
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cattle
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Cloning, Molecular
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DNA, Complementary / genetics
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Enzyme Activation
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Golgi Apparatus / metabolism
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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N-Ethylmaleimide-Sensitive Proteins
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Qa-SNARE Proteins
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Qb-SNARE Proteins
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Vesicular Transport Proteins*
Substances
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Autophagy-Related Protein 8 Family
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Carrier Proteins
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DNA, Complementary
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Gabarapl2 protein, mouse
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Gosr1 protein, mouse
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Qa-SNARE Proteins
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Qb-SNARE Proteins
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Recombinant Proteins
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Vesicular Transport Proteins
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Adenosine Triphosphatases
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N-Ethylmaleimide-Sensitive Proteins
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Nsf protein, mouse