Two new sterols, (22E)-23-methylergosta-5,7,22-trien-beta-ol (1) and 5alpha,6alpha-epoxy-(22E)-ergosta-8,14,22-triene -3beta,7alpha-diol (2), have been isolated from two edible mushrooms, Lentinula edodes and Tricholoma matsutake, respectively, together with twelve known ones (3-14). The structures of the new compounds were elucidated on the basis of their spectral data.