Abstract
We isolated a novel gene designated spinal cord-derived growth factor (SCDGF). Its expression was increased in chick spinal cords with embryonic development and decreased after hatching. The amino acid sequences of chick and human SCDGFs revealed a putative signal sequence followed by a CUB domain and a region homologous to the members of the platelet-derived growth factor/vascular endothelial growth factor family. Furthermore, human SCDGF secreted from the cells showed a mitogenic activity for 10T1/2 cells in vitro. These results led us to speculate that SCDGF plays an important role in the development of the spinal cord.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Northern
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CHO Cells
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Cell Survival / drug effects
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Chick Embryo
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Cloning, Molecular
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Cricetinae
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DNA, Complementary / metabolism
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Endothelial Growth Factors / genetics
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Humans
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Lymphokines / biosynthesis
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Lymphokines / chemistry
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Lymphokines / genetics*
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Microscopy, Phase-Contrast
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Molecular Sequence Data
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Multigene Family
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Platelet-Derived Growth Factor / biosynthesis
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Platelet-Derived Growth Factor / chemistry
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Platelet-Derived Growth Factor / genetics*
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Protein Structure, Tertiary
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Spinal Cord / embryology
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Spinal Cord / metabolism*
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Time Factors
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Transfection
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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DNA, Complementary
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Endothelial Growth Factors
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Lymphokines
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Platelet-Derived Growth Factor
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RNA, Messenger
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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platelet-derived growth factor C
Associated data
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GENBANK/AB033829
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GENBANK/AB033831