Abstract
Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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Antibody Specificity
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Cattle / genetics*
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Cattle / immunology*
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DNA, Complementary / genetics*
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Evolution, Molecular
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Genes, MHC Class II*
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HLA-DR Antigens / genetics*
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HLA-DRB3 Chains
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Haplotypes
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Histocompatibility Antigens Class II / genetics*
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Molecular Sequence Data
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Sequence Analysis, Protein
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Sequence Homology, Amino Acid
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Transfection
Substances
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Antibodies, Monoclonal
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DNA, Complementary
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HLA-DR Antigens
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HLA-DRB3 Chains
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Histocompatibility Antigens Class II
Associated data
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GENBANK/Y18246
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GENBANK/Y18308