Abstract
A 130-kDa glycoprotein (p130) has been found to be associated with surrogate light chain on pro- and pre-B I cells. Using peptide sequences obtained from purified p130 we have cloned its gene. The gene encodes a typical cadherin type 1 membrane protein with six extracellular cadherin domains (one pseudo domain) but lacking the catenin-binding site in its cytoplasmic part. Even without this catenin-binding site, p130 mediates Ca(2+)-dependent homotypic adhesion of cells. The interaction of p130 with surrogate light chain is confirmed by co-transfection and co-immunoprecipitation experiments. The expression of p130 is biphasic during the B cell development. Reverse transcriptase-polymerase chain reaction and flow cytometric analyses revealed that it is expressed on B220(+)c-Kit(+) pro-B and pre-B-I cells as well as on B220(+)CD25(-)IgM(+) immature and mature B cells but not on B220(+)CD25(+) pre-B-II cells. It is also expressed in fetal liver, at low levels in myeloid cells, and strongly in intestinal epithelial cells. In the spleen, p130-expressing cells are mainly localized in the marginal zone. We call this B lineage-, intestine-, liver- and leukocyte-expressed gene BILL-cadherin. The possible functions of BILL-cadherin in B cell development are discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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B-Lymphocytes / chemistry
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B-Lymphocytes / cytology*
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B-Lymphocytes / metabolism*
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Base Sequence
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Binding Sites
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Blotting, Northern
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Cadherins / biosynthesis*
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Cadherins / chemistry
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Cadherins / genetics*
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Calcium / metabolism
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Cell Adhesion
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Cell Line
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Cloning, Molecular
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DNA, Complementary / metabolism
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Drosophila
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Female
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Flow Cytometry
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Gene Library
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Immunoglobulin Light Chains / chemistry
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Immunoglobulin Light Chains, Surrogate
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Immunoglobulin M / metabolism
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Immunoglobulin Variable Region / chemistry
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Intestinal Mucosa / metabolism
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Leukocyte Common Antigens / metabolism
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Leukocytes / metabolism
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Liver / metabolism
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Membrane Glycoproteins / chemistry*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Molecular Sequence Data
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Precipitin Tests
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Protein Structure, Tertiary
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Protein Tyrosine Phosphatase, Non-Receptor Type 1
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Proto-Oncogene Proteins c-kit / metabolism
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Rats
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Receptors, Interleukin-2 / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Time Factors
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Tissue Distribution
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Transfection
Substances
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Antibodies, Monoclonal
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Cadherins
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Cdh17 protein, mouse
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DNA, Complementary
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Immunoglobulin Light Chains
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Immunoglobulin Light Chains, Surrogate
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Immunoglobulin M
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Immunoglobulin Variable Region
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Membrane Glycoproteins
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Receptors, Interleukin-2
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Proto-Oncogene Proteins c-kit
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Leukocyte Common Antigens
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Protein Tyrosine Phosphatase, Non-Receptor Type 1
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Calcium