Abstract
Anabaenopeptins I (1) and J (2), two new ureido bond-containing cyclic peptides, were isolated from the cultured cyanobacterium Aphanizomenon flos-aquae (NIES-81) as potent carboxypeptidase-A (CPA) inhibitors. The gross structures of 1 and 2 were established by spectroscopic analysis, including the 2D NMR techniques. The absolute configurations of 1 and 2 were determined by spectral and chemical methods. Anabaenopeptins I and J inhibited CPA with IC(50) values of 5.2 and 7.6 ng/mL, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carboxypeptidases / antagonists & inhibitors*
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Carboxypeptidases A
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Cyanobacteria / chemistry*
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification*
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Enzyme Inhibitors / pharmacology
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Peptides, Cyclic / chemistry
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Peptides, Cyclic / isolation & purification*
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Peptides, Cyclic / pharmacology
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Protein Conformation
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Spectrum Analysis
Substances
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Enzyme Inhibitors
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Peptides, Cyclic
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Carboxypeptidases
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Carboxypeptidases A