Abstract
The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells / drug effects
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Amino Acid Sequence
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Animals
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Base Sequence
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Betacellulin
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COS Cells
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Cloning, Molecular
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DNA, Complementary / analysis
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Epidermal Growth Factor / chemistry
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Gene Expression
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Growth Substances / genetics*
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Growth Substances / pharmacology
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Humans
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Intercellular Signaling Peptides and Proteins*
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Kidney / physiology
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Mice
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Mitogens / pharmacology
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Molecular Sequence Data
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Rats
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
Substances
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BTC protein, human
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Betacellulin
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Btc protein, mouse
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Btc protein, rat
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DNA, Complementary
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Mitogens
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Epidermal Growth Factor