Abstract
[structure] The biochemical induction assay (BIA) is a rapid (colorimetric) bacterial assay used to identify compounds that damage DNA or inhibit DNA synthesis and thereby identify potential natural product anticancer agents. Bisanthraquinones based on a 1,3,6, 8-tetrahydroxyanthraquinone-type carbon skeleton were isolated from an endophytic fungus and characterized by NMR and X-ray crystallography. Cytoskyrin A (1) is highly active in the biochemical induction assay, while the closely related cytoskyrin B (2) has no detectable activity in this assay.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anthraquinones / chemistry
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Anthraquinones / isolation & purification*
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Anthraquinones / pharmacology
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Antibiotics, Antineoplastic / chemistry
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Antibiotics, Antineoplastic / isolation & purification*
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Antibiotics, Antineoplastic / pharmacology
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Colorimetry
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Crystallography, X-Ray
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DNA, Bacterial / biosynthesis
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Magnetic Resonance Spectroscopy
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Mitosporic Fungi / chemistry*
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Models, Molecular
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Stereoisomerism
Substances
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Anthraquinones
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Antibiotics, Antineoplastic
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DNA, Bacterial
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cytoskyrin A
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cytoskyrin B