Abstract
The SHL gene from Arabidopsis thaliana encodes a small nuclear protein that contains a BAH domain and a PHD finger. Both domains are found in numerous (putative) transcriptional regulators and chromatin-remodeling factors. Different sets of transgenic lines were established to analyze the physiological relevance of SHL. SHL expression driven by the CaMV 35S promoter results in reduced growth, early flowering, early senescence, and impaired flower and seed formation. Antisense inhibition of SHL expression gives rise to dwarfism and delayed development. In-frame N-terminal fusion of the SHL protein to beta-glucuronidase (GUS) directs GUS to the nucleus of stably transformed Arabidopsis plants. Thus, SHL encodes a novel putative regulator of gene expression, which directly or indirectly influences a broad range of developmental processes.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics*
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Arabidopsis / growth & development*
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Arabidopsis / physiology
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Arabidopsis Proteins*
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Base Sequence
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DNA Primers / genetics
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DNA, Complementary / genetics
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DNA, Plant / genetics
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Fertility / genetics
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Plant
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Genes, Plant
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Glucuronidase / genetics
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Nuclear Proteins / physiology
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Plant Proteins / genetics*
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Plant Proteins / physiology
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Plants, Genetically Modified
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Plant / genetics
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RNA, Plant / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Arabidopsis Proteins
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DNA Primers
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DNA, Complementary
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DNA, Plant
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Nuclear Proteins
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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Recombinant Fusion Proteins
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SHL protein, Arabidopsis
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Glucuronidase