Abstract
The G protein beta3 subunit (GNB3) 825T allele is predictive of enhanced Gi protein activation. Studying the influence of C825T allele status on cellular in vitro immune responses towards recall antigens and interleukin-2 stimulation we observed a 2-4-fold, significantly increased proliferation in homozygous 825T (TT) vs. C825 allele (CC) carriers. Furthermore, lymphocyte chemotaxis and CD4(+) T cell counts of individuals with TT+TC genotypes were significantly enhanced compared to the CC genotype. In summary, it appears that C825T allele status is highly predictive of immunocompetence and could be a candidate gene in disorders associated with inadequate immune response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles*
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Antigens / pharmacology
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Antigens, CD / metabolism
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CD4 Lymphocyte Count
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Cell Division / drug effects
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Cell Division / immunology
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Chemotaxis / drug effects
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Female
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Genetic Markers
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Genotype
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Heterotrimeric GTP-Binding Proteins / genetics*
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Heterozygote
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Homozygote
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Humans
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Immunocompetence / genetics*
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Immunophenotyping
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Interleukin-2 / pharmacology
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / genetics*
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Lymphocyte Subsets / cytology
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Lymphocyte Subsets / metabolism
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Male
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
Substances
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Antigens
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Antigens, CD
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Genetic Markers
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Interleukin-2
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Heterotrimeric GTP-Binding Proteins