NoRC--a novel member of mammalian ISWI-containing chromatin remodeling machines

R Strohner; A Nemeth; P Jansa; U Hofmann-Rohrer; R Santoro; G Längst; I Grummt

doi:10.1093/emboj/20.17.4892

NoRC--a novel member of mammalian ISWI-containing chromatin remodeling machines

EMBO J. 2001 Sep 3;20(17):4892-900. doi: 10.1093/emboj/20.17.4892.

Authors

R Strohner¹, A Nemeth, P Jansa, U Hofmann-Rohrer, R Santoro, G Längst, I Grummt

Affiliation

¹ Division of Molecular Biology of the Cell II, Deutsches Krebsforschungszentrum, D-69120 Heidelberg and Adolf-Butenandt-Institut, Schillerstrasse 44, D-80336 München, Germany.

Abstract

Transcription by RNA polymerase I on nucleosomal templates requires binding of the transcription termination factor TTF-I to a cognate site 160 bp upstream of the transcription start site. Binding of TTF-I is accompanied by changes in the chromatin architecture which suggests that TTF-I recruits a remodeling activity to the rDNA promoter. We have cloned a cDNA that encodes TIP5 (TTF-I-interacting protein 5), a 205 kDa protein that shares a number of important protein domains with WSTF (Williams syndrome transcription factor) and hAcf1/WCRF180, the largest subunits of human chromatin remodeling complexes hCHRAC and WCRF. TIP5 co-localizes with the basal RNA polymerase I transcription factor UBF in the nucleolus and is associated with SNF2h. The cellular TIP5-SNF2h complex, termed NoRC (nucleolar remodeling complex), induces nucleosome sliding in an ATP- and histone H4 tail-dependent fashion. The results suggest that NoRC is a novel nucleolar chromatin remodeling machine that may serve a role in the regulation of the rDNA locus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Adenosine Triphosphatases / chemistry
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism*
Amino Acid Sequence
Animals
Cell Line
Chromatin / genetics*
Chromatin / metabolism
Chromosomal Proteins, Non-Histone / chemistry
Chromosomal Proteins, Non-Histone / genetics*
Chromosomal Proteins, Non-Histone / metabolism*
Cloning, Molecular
DNA-Binding Proteins / metabolism
Humans
Mammals
Mice
Molecular Sequence Data
Open Reading Frames
Promoter Regions, Genetic
Recombinant Fusion Proteins / metabolism
Recombinant Proteins / metabolism
Saccharomyces cerevisiae
Sequence Alignment
Sequence Homology, Amino Acid
Transcription Factors / chemistry
Transcription Factors / genetics*
Transcription Factors / metabolism*
Transfection

Substances

BAZ2A protein, human
Baz2a protein, mouse
Chromatin
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
ISWI protein
Recombinant Fusion Proteins
Recombinant Proteins
TTF1 protein, human
Transcription Factors
Ttf1 protein, mouse
Adenosine Triphosphatases
SMARCA5 protein, human
Smarca5 protein, mouse