Abstract
Leg muscle was biopsied and frozen for storage at -70 degrees C. from 5 wild-type mice, two knocked out acid alpha-glucosidase (GAA) gene mice, and seven glycogen synthase plus glucose muscle transporter transgenic mice. All of the wild-type mice had very little muscle glycogen (3.58 +/- 1.67 micromols glucosyl subunits per g muscle), and 52% or more of its glycogen phosphorylase activity without AMP (69% +/- 17% glycogen phosphorylase a). In contrast the GAA knockout and transgenic mice had glycogen ranging from 63 to 297 micromols glucosyl subunits per g muscle, and very little or no glycogen phosphorylase activity without 1.00 mM AMP (4.8% and less glycogen phosphorylase a). This suggests that there is an inverse relationship between mouse muscle phosphorylase a and the muscle's glycogen content.
MeSH terms
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Animals
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Enzyme Activation / physiology
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Fructose-Bisphosphate Aldolase / metabolism
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Glucan 1,4-alpha-Glucosidase / deficiency
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Glucan 1,4-alpha-Glucosidase / genetics
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Glucan 1,4-alpha-Glucosidase / metabolism
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Glucose Transporter Type 1
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Glycogen / analysis
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Glycogen / metabolism*
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Glycogen Debranching Enzyme System / metabolism
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Glycogen Synthase / genetics
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Glycogen Synthase / metabolism
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Mice
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Mice, Knockout
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Mice, Transgenic
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Monosaccharide Transport Proteins / genetics
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Monosaccharide Transport Proteins / metabolism
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Muscle, Skeletal / chemistry
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Muscle, Skeletal / metabolism*
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Phosphofructokinases / metabolism
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Phosphorylase a / metabolism*
Substances
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Glucose Transporter Type 1
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Glycogen Debranching Enzyme System
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Monosaccharide Transport Proteins
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Glycogen
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Phosphorylase a
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Glycogen Synthase
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Phosphofructokinases
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Glucan 1,4-alpha-Glucosidase
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Fructose-Bisphosphate Aldolase