Abstract
Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-I' from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-I' under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-I' does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins indicating that its antiviral activity most probably differs from that of pokeweed antiviral protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiviral Agents / chemistry
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Antiviral Agents / pharmacology*
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Gene Expression
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N-Glycosyl Hydrolases / chemistry
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N-Glycosyl Hydrolases / genetics
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N-Glycosyl Hydrolases / pharmacology*
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Nicotiana / drug effects*
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Nicotiana / virology*
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Plant Diseases / virology
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / pharmacology*
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Plants, Genetically Modified
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Sambucus nigra / genetics
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Tobacco Mosaic Virus
Substances
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Antiviral Agents
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Plant Proteins
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Recombinant Proteins
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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N-Glycosyl Hydrolases
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pokeweed antiviral protein