Phylogenetic analysis of a human isolate from the 2000 Israel West Nile virus epidemic

Thomas Briese; Andrew Rambaut; Melissa Pathmajeyan; Jihad Bishara; Miriam Weinberger; Silvio Pitlik; W Ian Lipkin

doi:10.3201/eid0805.010324

Phylogenetic analysis of a human isolate from the 2000 Israel West Nile virus epidemic

Emerg Infect Dis. 2002 May;8(5):528-31. doi: 10.3201/eid0805.010324.

Authors

Thomas Briese¹, Andrew Rambaut, Melissa Pathmajeyan, Jihad Bishara, Miriam Weinberger, Silvio Pitlik, W Ian Lipkin

Affiliation

¹ Emerging Diseases Laboratory, Dept. of Neurology, Microbiology and Molecular Genetics, 3107 Gillespie Neuroscience Building, University of California at Irvine, Irvine, CA 92697-4292, USA. tbriese@uci.edu

Abstract

Specimens from a patient of the 2000 Israel West Nile virus epidemic were analyzed by reverse transcription-polymerase chain reaction. Products corresponding to E, NS3, and NS5 sequences were amplified from cerebellar but not from cortical samples. Phylogenetic analyses indicated a closer relationship of this isolate to 1996 Romanian and 1999 Russian than to 1998-99 Israeli or 1999 New York isolates.

Publication types

Case Reports
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Aged
Disease Outbreaks
Fatal Outcome
Female
Humans
Israel
Molecular Sequence Data
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
West Nile Fever / virology*
West Nile virus / genetics*
West Nile virus / isolation & purification*

Associated data

GENBANK/AF394217

Grants and funding

NS 29425/NS/NINDS NIH HHS/United States