Abstract
Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacteriophage N4 / enzymology*
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Bacteriophage N4 / physiology
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DNA-Directed RNA Polymerases / classification
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DNA-Directed RNA Polymerases / genetics
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Dimerization
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Escherichia coli / growth & development
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Escherichia coli / virology*
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Molecular Sequence Data
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RNA Polymerase II / chemistry*
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RNA Polymerase II / genetics*
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RNA Polymerase II / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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Recombinant Fusion Proteins
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Viral Proteins
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RNA Polymerase II
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DNA-Directed RNA Polymerases
Associated data
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GENBANK/AY074660
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GENBANK/AY074661