Identification of ribosomal proteins specific to higher eukaryotic organisms

Cyril Gueydan; Corinne Wauquier; Christelle De Mees; Georges Huez; Véronique Kruys

doi:10.1074/jbc.M208551200

Identification of ribosomal proteins specific to higher eukaryotic organisms

J Biol Chem. 2002 Nov 22;277(47):45034-40. doi: 10.1074/jbc.M208551200. Epub 2002 Aug 28.

Authors

Cyril Gueydan¹, Corinne Wauquier, Christelle De Mees, Georges Huez, Véronique Kruys

Affiliation

¹ Laboratoire de Chimie Biologique, Institut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, 12 rue des Profs. Jeener et Brachet, 6041 Gosselies, Belgium. cgueydan@ulb.ac.be

PMID: 12202495
DOI: 10.1074/jbc.M208551200

Abstract

This report describes the identification of a novel protein named PS1D (Genbank accession number ), which is composed of an S1-like RNA-binding domain, a (cysteine)x3-(histidine) CCCH-zinc finger, and a very basic carboxyl domain. PS1D is expressed as two isoforms, probably resulting from the alternative splicing of mRNA. The long PS1D isoform differs from the short one by the presence of 48 additional amino acids at its amino-terminal extremity. Analysis of PS1D subcellular distribution by cell fractionation reveals that this protein belongs to the core of the eukaryotic 60S ribosomal subunit. Interestingly, PS1D protein is a highly conserved protein among mammalians as murine, human, and simian PS1D homologues share more than 95% identity. In contrast, no homologous protein is found in lower eukaryotes such as yeast and Caenorhabditis elegans. These observations indicate that PS1D is the first eukaryotic ribosomal protein that is specific to higher eukaryotes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cell Fractionation
Cell Line
Genes, Reporter
Humans
Mice
Molecular Sequence Data
Nuclear Proteins / chemistry
Nuclear Proteins / genetics
Nuclear Proteins / isolation & purification
Nuclear Proteins / metabolism*
Protein Isoforms
RNA, Ribosomal / genetics
RNA, Ribosomal / metabolism
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / genetics
RNA-Binding Proteins / isolation & purification
RNA-Binding Proteins / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Ribosomal Proteins / chemistry
Ribosomal Proteins / genetics
Ribosomal Proteins / isolation & purification
Ribosomal Proteins / metabolism*
Ribosomes / chemistry*
Ribosomes / genetics
Ribosomes / metabolism
Sequence Alignment
Tissue Distribution

Substances

Nuclear Proteins
PS1D protein, mouse
Protein Isoforms
RNA, Ribosomal
RNA-Binding Proteins
Recombinant Fusion Proteins
Ribosomal Proteins

Associated data

GENBANK/AJ272345