Implication of the lymphocyte-specific nuclear body protein Sp140 in an innate response to human immunodeficiency virus type 1

J Virol. 2002 Nov;76(21):11133-8. doi: 10.1128/jvi.76.21.11133-11138.2002.

Abstract

The viral infectivity factor (Vif) of human immunodeficiency virus type 1 (HIV-1) neutralizes an unidentified antiviral pathway that occurs only in nonpermissive (NP) cells. Using a yeast two-hybrid screen of a human lymphocyte cDNA library, we identified several potential Vif partners. One, the nuclear body protein Sp140, was found specifically in all NP cells (n = 12 cell lines tested; P < or = 0.001), and HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif. Our results implicate Sp140 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Nuclear / genetics
  • Antigens, Nuclear / metabolism*
  • Binding Sites
  • Gene Products, vif / genetics
  • Gene Products, vif / metabolism*
  • HIV-1 / metabolism*
  • HL-60 Cells
  • HeLa Cells
  • Humans
  • Jurkat Cells
  • Lymphocytes / metabolism
  • Lymphocytes / virology
  • Mutagenesis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Tumor Cells, Cultured
  • U937 Cells
  • vif Gene Products, Human Immunodeficiency Virus

Substances

  • Antigens, Nuclear
  • Gene Products, vif
  • SP140 protein, human
  • Transcription Factors
  • vif Gene Products, Human Immunodeficiency Virus