Hispin, a novel ribosome inactivating protein with antifungal activity from hairy melon seeds

T B Ng; A Parkash

doi:10.1016/s1046-5928(02)00511-9

Hispin, a novel ribosome inactivating protein with antifungal activity from hairy melon seeds

Protein Expr Purif. 2002 Nov;26(2):211-7. doi: 10.1016/s1046-5928(02)00511-9.

Authors

T B Ng¹, A Parkash

Affiliation

¹ Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China. biochemistry@cuhk.edu.hk

PMID: 12406674
DOI: 10.1016/s1046-5928(02)00511-9

Abstract

A ribosome inactivating protein demonstrating a molecular mass of 21 kDa and a novel N-terminal sequence was isolated from seeds of the hairy melon. The purification procedure involved affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose and Mono S. The protein designated hispin inhibited translation in the cell-free rabbit reticulocyte lysate system with an IC50 of 165 pM and exhibited N-glycosidase activity. Antifungal activity was also observed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antifungal Agents / chemistry
Antifungal Agents / isolation & purification*
Antifungal Agents / pharmacology
Chromatography, Affinity
Cucumis / embryology*
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Plant Proteins / chemistry
Plant Proteins / isolation & purification*
Plant Proteins / pharmacology
Ribosomes / drug effects*
Seeds / chemistry*

Substances

Antifungal Agents
Plant Proteins