Semi quantitative expression analysis of MDR3, FIC1, BSEP, OATP-A, OATP-C,OATP-D, OATP-E and NTCP gene transcripts in 1st and 3rd trimester human placenta

P Patel; N Weerasekera; M Hitchins; C A R Boyd; D G Johnston; C Williamson

doi:10.1053/plac.2002.0879

Semi quantitative expression analysis of MDR3, FIC1, BSEP, OATP-A, OATP-C,OATP-D, OATP-E and NTCP gene transcripts in 1st and 3rd trimester human placenta

Placenta. 2003 Jan;24(1):39-44. doi: 10.1053/plac.2002.0879.

Authors

P Patel¹, N Weerasekera, M Hitchins, C A R Boyd, D G Johnston, C Williamson

Affiliation

¹ Imperial College School of Science, Technology and Medicine, Institute of Reproductive and Developmental Biology, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK.

PMID: 12495658
DOI: 10.1053/plac.2002.0879

Abstract

Using real time RT-PCR, we have detected expression of seven genes that influence bile acid transport,MDR3, FIC1, BSEP, OATP-A, OATP-C, OATP-D and OATP-E, in normal human placenta. With the exception of OATP-C and OATP-E these genes were found to be differentially expressed in 1st trimester and 3rd trimester placentae. MDR3 gene expression was found to be up regulated four fold in 3rd trimester placentae compared to 1st trimester, OATP-A gene expression was down regulated eight fold, OATP-D was down regulated 17 fold, while FIC1 expression was reduced by 33 fold in the 3rd trimester. OATP-C and BSEP gene expression was not detected in the 3rd trimester placenta, while low levels of transcripts were detected in the 1st trimester placentae. Transcripts of the hepatic sinusoidal bile acid transporter, NTCP, were not detected in placenta.

MeSH terms

ATP Binding Cassette Transporter, Subfamily B / genetics
ATP Binding Cassette Transporter, Subfamily B / metabolism
ATP Binding Cassette Transporter, Subfamily B, Member 11
ATP-Binding Cassette Transporters / genetics
ATP-Binding Cassette Transporters / metabolism
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / metabolism
Adult
Bile Acids and Salts / metabolism
Carrier Proteins / genetics*
Carrier Proteins / metabolism
Down-Regulation
Female
Gene Expression Regulation, Developmental*
Humans
Membrane Transport Proteins*
Organic Anion Transporters / genetics
Organic Anion Transporters / metabolism
Organic Anion Transporters, Sodium-Dependent
Placenta / metabolism*
Pregnancy
Pregnancy Trimester, First
Pregnancy Trimester, Third
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Symporters
Transcription, Genetic*
Up-Regulation

Substances

ABCB11 protein, human
ATP Binding Cassette Transporter, Subfamily B
ATP Binding Cassette Transporter, Subfamily B, Member 11
ATP-Binding Cassette Transporters
Bile Acids and Salts
Carrier Proteins
Membrane Transport Proteins
Organic Anion Transporters
Organic Anion Transporters, Sodium-Dependent
RNA, Messenger
Symporters
sodium-bile acid cotransporter
multidrug resistance protein 3
Adenosine Triphosphatases
ATP8B1 protein, human