Abstract
A new gene, sul3, which specifies a 263-amino-acid protein similar to a dihydropteroate synthase encoded by the 54-kb conjugative plasmid pVP440 from Escherichia coli was characterized. Expression of the cloned sul3 gene conferred resistance to sulfamethoxazole on E. coli. Two copies of the insertion element IS15Delta/26 flanked the region containing sul3. The sul3 gene was detected in one-third of the sulfonamide-resistant pathogenic E. coli isolates from pigs in Switzerland.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anti-Bacterial Agents / pharmacology*
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Carrier Proteins / genetics
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Chromosomes, Bacterial / drug effects
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Chromosomes, Bacterial / genetics
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Conjugation, Genetic
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DNA Transposable Elements / genetics
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Drug Resistance, Bacterial
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Escherichia coli / drug effects*
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Escherichia coli / genetics*
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Genes, Bacterial / genetics*
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Molecular Sequence Data
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Phenotype
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Plasmids / genetics
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Sulfonamides / pharmacology*
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Swine / microbiology*
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Switzerland
Substances
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Anti-Bacterial Agents
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Carrier Proteins
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DNA Transposable Elements
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Sulfonamides
Associated data
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GENBANK/AE000398
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GENBANK/AF483270
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GENBANK/AJ294721
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GENBANK/AJ459418