The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Vivi Joosten; Christien Lokman; Cees AMJJ Van Den Hondel; Peter J Punt

doi:10.1186/1475-2859-2-1

The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Microb Cell Fact. 2003 Jan 30;2(1):1. doi: 10.1186/1475-2859-2-1.

Authors

Vivi Joosten¹, Christien Lokman, Cees AMJJ Van Den Hondel, Peter J Punt

Affiliation

¹ TNO Nutrition and Food Research, Department of Applied Microbiology and Gene Technology, P,O, Box 360, 3700 AJ Zeist, The Netherlands. joosten@voeding.tno.nl

Abstract

In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of fragments to relevant enzymes or other components will be discussed. As an example of the fusion protein strategy, the 'magic bullet' approach for industrial applications, will be highlighted.