Abstract
Mouse Spot-1 is a DNA-binding protein with a domain (His-Thr) encoded by p(CA)n repeats. Spot-1 interacts with the nuclear localization signal (NLS) I of p53 through its His-Thr domain. In this study we describe the cloning and expression patterns of a novel gene encoding a protein containing a His-Thr domain, Spot-2. Spot-2 is exclusively expressed in the pituitary from stage E13.5 to E15.5. Mouse Lhx3 plays a critical role during early organogenesis in the pituitary. The Spot-2 gene appears to be a downstream gene of Lhx3. It is suggested that Spot-2 plays important roles in pituitary development.
MeSH terms
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Amino Acid Sequence
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Animals
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Developmental*
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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LIM-Homeodomain Proteins
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Mice
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Mice, Mutant Strains
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Molecular Sequence Data
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Nuclear Localization Signals
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Pituitary Gland / embryology*
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Pituitary Gland / physiology
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Protein Structure, Tertiary
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Sequence Homology, Amino Acid
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Transcription Factors
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Tumor Suppressor Protein p53 / metabolism
Substances
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DNA-Binding Proteins
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Homeodomain Proteins
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LIM-Homeodomain Proteins
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Lhx3 protein
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Nuclear Localization Signals
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Spot-2 protein, mouse
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Transcription Factors
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Tumor Suppressor Protein p53