Sequence analysis demonstrates that Onion yellow dwarf virus isolates from China contain a P3 region much larger than other potyviruses

J Chen; M J Adams; H-Y Zheng; J-P Chen

doi:10.1007/s00705-003-0020-1

Sequence analysis demonstrates that Onion yellow dwarf virus isolates from China contain a P3 region much larger than other potyviruses

Arch Virol. 2003 Jun;148(6):1165-73. doi: 10.1007/s00705-003-0020-1.

Authors

J Chen¹, M J Adams, H-Y Zheng, J-P Chen

Affiliation

¹ Virology and Biotechnology Department, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.

PMID: 12756621
DOI: 10.1007/s00705-003-0020-1

Abstract

The complete sequence of an isolate of Onion yellow dwarf virus (OYDV) from Yuhang, Zhejiang province, China, was determined. It was 10538 nts in length and was predicted to encode a polyprotein 3403 amino acids (aa) long with a calculated M(r) of 385.1 kDa. The predicted P3 protein (530 aa) was larger than that of any of the potyviruses sequenced to date (344-378 aa). The additional sequence occurs at the N-terminus of the protein, does not represent a duplication from elsewhere in the OYDV genome and could not be matched to any other sequences in the databases. Similar sequences were found in 4 other Chinese OYDV isolates. Phylogenetic analysis of the amino acid sequences of the polyprotein showed that OYDV is distantly related to Pea seed-borne mosaic virus and the potyviruses of grasses and cereals.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
China
Molecular Sequence Data
Onions / virology*
Phylogeny
Potyviridae / classification*
Potyviridae / genetics*
Sequence Alignment
Sequence Analysis, DNA
Viral Proteins / chemistry
Viral Proteins / genetics

Substances

Viral Proteins