Abstract
Interleukin 8 (IL-8) is a chemotactic and activating chemokine, especially for neutrophils, which plays an important role in inflammatory process. A pig IL-8 specific enzyme-linked immunosorbent assay (ELISA) was developed to measure IL-8 concentrations in cell culture supernatants and biological fluids. A streptavidin-biotin amplified sandwich method uses mouse capture mAb IZ8.03 and detection biotinylated mouse mAb IZ8.04 against recombinant pig IL-8. The assay specifically and reproducibly recognizes both recombinant and natural pig IL-8. A working range of the assay is 16-1000 pg/mL and takes a mere 3.5 h of incubation time. This pig IL-8 ELISA is a suitable alternative way of measurement of IL-8 concentrations to time consuming and laborious IL-8 bioassays.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amniotic Fluid / chemistry
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Amniotic Fluid / immunology
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Animals
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / immunology
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Antibody Specificity
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Biotin / chemistry
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Biotin / immunology
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Cells, Cultured
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Cytokines / analysis
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Cytokines / immunology
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Enzyme-Linked Immunosorbent Assay / methods*
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Escherichia coli / genetics
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Escherichia coli / immunology
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Escherichia coli / pathogenicity
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Fetus / immunology
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Fetus / microbiology
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Interleukin-8 / analysis*
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Interleukin-8 / immunology
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Leukocytes, Mononuclear / immunology
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Mice
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Mice, Inbred BALB C
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Recombinant Proteins / analysis
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Recombinant Proteins / immunology
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Sensitivity and Specificity
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Streptavidin / chemistry
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Streptavidin / immunology
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Swine / blood
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Swine / immunology*
Substances
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Antibodies, Monoclonal
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Cytokines
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Interleukin-8
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Recombinant Proteins
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Biotin
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Streptavidin