Abstract
Rat ERK6, also known as SAPK3 and p38gamma, exhibits a distinct pattern of expression during muscle development in vitro and in vivo. Levels of mRNA transcript and protein abundance for ERK6 are increased during the differentiation of 2 rodent myoblast cell lines in culture. This is in contrast to the expression of other MAP kinase family members, namely p42/p44 MAPK and p38 MAPK, whose expression does not change during myogenesis. Similar results are observed in vivo where ERK6 mRNA levels increase with post-natal development in rat hindlimb mixed muscle samples. These results delineate a distinct pattern of ERK6 expression in mature skeletal muscle cells and suggest a specific role for ERK6 in muscle development or muscle function.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Differentiation
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Cell Line
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Cloning, Molecular
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Enzymologic
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MAP Kinase Signaling System
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Mitogen-Activated Protein Kinase 1 / genetics
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Mitogen-Activated Protein Kinase 12
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases / genetics*
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Muscle, Skeletal / enzymology*
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Muscle, Skeletal / growth & development*
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Myoblasts / cytology
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Myoblasts / enzymology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Tissue Distribution
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p38 Mitogen-Activated Protein Kinases
Substances
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RNA, Messenger
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Mitogen-Activated Protein Kinase 12
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
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p38 Mitogen-Activated Protein Kinases