Abstract
We recently identified and cloned a novel human gene, Che-1, whose product interacts with both RNA polymerase II and the retinoblastoma gene product (Rb). Furthermore, we found that Che-1 overexpression counteracts the growth inhibitory effects of Rb, regulating in such way both transcription and cell proliferation. In this paper, we describe the genomic organization of the mouse orthologous Che-1 gene and its promoter region. The promoter is TATA less and presents several potential transcription factor-binding motifs. Importantly, we showed that Che-1 expression is regulated by a negative feedback mechanism, in which this protein is present on its own promoter repressing transcription.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Apoptosis Regulatory Proteins
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Base Sequence
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Cells, Cultured
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Cloning, Molecular
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DNA / chemistry
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DNA / genetics
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Exons
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Female
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Gene Expression Regulation
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Genes / genetics*
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Humans
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Introns
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Luciferases / genetics
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Luciferases / metabolism
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Male
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Mice
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Molecular Sequence Data
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NIH 3T3 Cells
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Promoter Regions, Genetic / genetics
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Protein Binding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Retinoblastoma Protein / metabolism*
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Sequence Analysis, DNA
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription, Genetic
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Transfection
Substances
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AATF protein, human
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Apoptosis Regulatory Proteins
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DNA, Complementary
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RNA, Messenger
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Recombinant Fusion Proteins
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Repressor Proteins
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Retinoblastoma Protein
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Transcription Factors
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DNA
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Luciferases
Associated data
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GENBANK/AF322223
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GENBANK/AY306199