Molecular cloning and characterization of three adult rat beta-globin gene promoters

Satoshi Okumura; Hiroyuki Fujii; Naomi Inokuchi; Michiaki Watanabe; Takeshi Nishino; Taro Okazaki

doi:10.1016/j.bbaexp.2004.02.002

Molecular cloning and characterization of three adult rat beta-globin gene promoters

Biochim Biophys Acta. 2004 May 25;1678(2-3):145-9. doi: 10.1016/j.bbaexp.2004.02.002.

Authors

Satoshi Okumura¹, Hiroyuki Fujii, Naomi Inokuchi, Michiaki Watanabe, Takeshi Nishino, Taro Okazaki

Affiliation

¹ Department of First Internal Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo, Tokyo 113-8602, Japan.

PMID: 15157740
DOI: 10.1016/j.bbaexp.2004.02.002

Abstract

We analyzed the promoter regions of the adult rat beta (IIbeta, IIIbeta, and 0beta)-globin genes. The results indicated that (1) the activities of the minimal promoters of these three genes are proportional to the gene expression levels in vivo, and (2) erythroid-specific repressor regions are located immediately upstream of the minimal promoter sequences and are regulated by the same transcription factor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cells, Cultured
Chloramphenicol O-Acetyltransferase / metabolism
Cloning, Molecular
DNA / chemistry
Enhancer Elements, Genetic
Gene Expression Regulation
Gene Library
Globins / genetics*
HeLa Cells
Humans
Mice
Models, Genetic
Molecular Sequence Data
Promoter Regions, Genetic*
Rats
Transcription Factors
Transfection

Substances

Transcription Factors
Globins
DNA
Chloramphenicol O-Acetyltransferase

Associated data

GENBANK/S74802
GENBANK/X67614
GENBANK/X67615
GENBANK/X67616