Abstract
Hemokinin 1 is encoded by preprotachykinin C (PPT-C) mRNA, and has been proposed as a regulator of B and T cell lymphopoiesis. Here we demonstrate the expression of mouse PPT-C mRNA by CD11b+ macrophages, CD11c+ dendritic cells and in the microglial cell line EOC 13.31. Expression was detected in freshly isolated CD11b+CD11c+ bone marrow cells, as well as in M-CSF expanded bone marrow-derived macrophages and GM-CSF expanded bone marrow-derived dendritic cells. There was preferential expression of PPT-C mRNA in dendritic cell subpopulations that were CD11b+, but not B220+ or GR-1+. These studies are the first to demonstrate PPT-C mRNA expression by cells of the myeloid lineage.
Copyright 2004 Elsevier B.V.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology
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CD11 Antigens / immunology
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Cell Differentiation / drug effects
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Cell Differentiation / genetics*
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Cell Differentiation / immunology
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Cell Lineage / drug effects
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Cell Lineage / genetics
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Cell Lineage / immunology
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Cells, Cultured
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Female
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
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Leukocyte Common Antigens / immunology
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Macrophages / drug effects
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Macrophages / immunology
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Macrophages / metabolism
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Mice
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Mice, Inbred C57BL
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Microglia / drug effects
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Microglia / immunology
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Microglia / metabolism
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Myeloid Cells / drug effects
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Myeloid Cells / immunology*
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Myeloid Cells / metabolism
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Myelopoiesis / drug effects
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Myelopoiesis / genetics*
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Myelopoiesis / immunology
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Protein Precursors / genetics*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism*
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Tachykinins / genetics*
Substances
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CD11 Antigens
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Protein Precursors
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RNA, Messenger
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Tac4 protein, mouse
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Tachykinins
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preprotachykinin
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Granulocyte-Macrophage Colony-Stimulating Factor
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Leukocyte Common Antigens