Abstract
Phenotypic changes in injured livers involve complex network of genes whose interplays may lead to fibrosis and cirrhosis, a major risk of hepatocellular carcinoma. Gene expression profiles in fibrotic livers were analyzed by using cDNA microarray, hierarchical clustering and gene ontology. Analyses of a major cluster of upregulated genes in cirrhosis identified a new set of genes involved in DNA repair and damage. The upregulation of DNA repair genes was confirmed by real-time quantitative polymerase chain reaction and associated with necroinflammatory activity (P<0.001). Increased DNA repair activity in cirrhosis with inflammatory activity may reflect increased DNA damages as a consequence of chronic liver injury.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Carcinoma, Hepatocellular / etiology*
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / metabolism
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DNA Repair / genetics*
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Endonucleases / analysis
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Endonucleases / genetics
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Endonucleases / metabolism
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Endothelial Cells / immunology
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Endothelial Cells / pathology
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Female
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Humans
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Liver / immunology
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Liver / pathology
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Liver Cirrhosis / complications*
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Liver Cirrhosis / etiology
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Liver Cirrhosis / genetics*
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Liver Cirrhosis / metabolism
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Liver Neoplasms / etiology*
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Liver Neoplasms / genetics
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Liver Neoplasms / metabolism
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Male
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Middle Aged
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Oligonucleotide Array Sequence Analysis
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Up-Regulation*
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Xeroderma Pigmentosum Group A Protein
Substances
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DNA-Binding Proteins
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XPA protein, human
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Xeroderma Pigmentosum Group A Protein
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ERCC1 protein, human
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Endonucleases