The genome of the Pseudomonas aeruginosa generalized transducing bacteriophage F116

Gene. 2005 Feb 14:346:187-94. doi: 10.1016/j.gene.2004.11.001. Epub 2005 Jan 28.

Abstract

F116 is a temperate, pilus-specific, generalized transducing phage belonging to the Podoviridae virus family. Its genome is linear, ds, TR, and CP DNA with a GC content of 63.2%. The 65 195-bp genome contains 70 putative ORFs, only 16 of which showed sequence similarity to Pseudomonas genomic or phage genes. While the current literature suggests that F116 is a non-integrating phage that maintains itself as a plasmid during the lysogenic life cycle, a putative int gene was identified. Of the phage structural genes, only the portal protein could be identified by homology. Analysis of F116 structural protein by one-dimensional SDS-PAGE revealed approximately 15 bands. MALDI-TOF MS analysis identified the gene encoding the major capsid protein. This protein appears to undergo posttranslational cleavage giving rise to a smaller capsid protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / genetics*
  • DNA Replication
  • Electrophoresis, Polyacrylamide Gel
  • Evolution, Molecular
  • Genome, Bacterial*
  • Plasmids
  • Pseudomonas aeruginosa / genetics*
  • Recombination, Genetic
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization