Detection of a novel carbon-phosphorus bond cleavage activity in cell-free extracts of an environmental Pseudomonas fluorescens isolate

G McMullan; J P Quinn

doi:10.1016/0006-291x(92)90693-f

Detection of a novel carbon-phosphorus bond cleavage activity in cell-free extracts of an environmental Pseudomonas fluorescens isolate

Biochem Biophys Res Commun. 1992 Apr 30;184(2):1022-7. doi: 10.1016/0006-291x(92)90693-f.

Authors

G McMullan¹, J P Quinn

Affiliation

¹ School of Biology and Biochemistry, Queen's University, Belfast, Northern Ireland.

PMID: 1575721
DOI: 10.1016/0006-291x(92)90693-f

Abstract

Cell-free extracts of Pseudomonas fluorescens strain 23F catalyzed the hydrolysis of phosphonoacetate to acetate and inorganic phosphate; the products were detected in almost equimolar quantities. The stable in vitro activity responsible was distinct from phosphonoacetaldehyde hydrolase and appears to represent a novel mode of carbon-phosphorus bond cleavage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetates / metabolism
Cell-Free System
Hydrolases / metabolism
Hydrolysis
Kinetics
Phosphates / metabolism
Phosphonoacetic Acid / metabolism*
Pseudomonas fluorescens / growth & development
Pseudomonas fluorescens / metabolism*

Substances

Acetates
Phosphates
Hydrolases
phosphonoacetaldehyde hydrolase
Phosphonoacetic Acid