Genomic DNA amplification from a single bacterium

Appl Environ Microbiol. 2005 Jun;71(6):3342-7. doi: 10.1128/AEM.71.6.3342-3347.2005.

Abstract

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacillus Phages / enzymology
  • Base Sequence
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics*
  • DNA-Directed DNA Polymerase / metabolism
  • Escherichia coli K12 / cytology*
  • Escherichia coli K12 / genetics
  • Flow Cytometry
  • Genetic Techniques
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Myxococcus xanthus / cytology
  • Myxococcus xanthus / genetics
  • Nucleic Acid Amplification Techniques / methods*
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • DNA-Directed DNA Polymerase