Abstract
Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacillus Phages / enzymology
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Base Sequence
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics*
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DNA-Directed DNA Polymerase / metabolism
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Escherichia coli K12 / cytology*
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Escherichia coli K12 / genetics
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Flow Cytometry
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Genetic Techniques
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Genome, Bacterial*
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Molecular Sequence Data
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Myxococcus xanthus / cytology
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Myxococcus xanthus / genetics
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Nucleic Acid Amplification Techniques / methods*
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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RNA, Ribosomal, 16S
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DNA-Directed DNA Polymerase