The rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair

Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4952-6. doi: 10.1073/pnas.89.11.4952.

Abstract

A number of important molecular checkpoints are believed to control the orderly progression of cell cycle events. We have found that the radiation-sensitive Schizosaccharomyces pombe mutant rad3-136 is deficient in two molecular checkpoint functions. Unlike wild-type cells, the mutant cells are unable to arrest in the G2 phase of the cell cycle after DNA damage by gamma-irradiation and are also incapable of maintaining the dependence of mitosis upon the completion of DNA synthesis. An S. pombe genomic clone that complements the UV sensitivity of the rad3-136 mutant completely restores the missing checkpoint functions. The rad3+ gene is also likely to play a role in DNA repair.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Benomyl / pharmacology
  • Cell Cycle*
  • Cloning, Molecular
  • DNA Ligases / metabolism
  • DNA Repair*
  • DNA Replication
  • Gamma Rays
  • Genes, Fungal*
  • Genetic Complementation Test
  • Hydroxyurea / pharmacology
  • Mitosis
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / genetics*

Substances

  • DNA Ligases
  • Benomyl
  • Hydroxyurea