The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

Anal Biochem. 2005 Sep 1;344(1):141-3. doi: 10.1016/j.ab.2005.05.022.

Authors

K Dheda¹, J F Huggett, J S Chang, L U Kim, S A Bustin, M A Johnson, G A W Rook, A Zumla

Affiliation

¹ Centre for Infectious Diseases and International Health, Royal Free and University College Medical School, and Department of Thoracic and HIV Medicine, Royal Free Hospital, London NW3 2QG, UK.

PMID: 16054107
DOI: 10.1016/j.ab.2005.05.022

No abstract available

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Diagnostic Errors / prevention & control
Gene Expression Profiling
Genes*
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
Humans
Interleukin-4 / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / standards*
Ribosomal Proteins / genetics
Toll-Like Receptor 2 / biosynthesis
Tuberculosis, Pulmonary / metabolism

Substances

Ribosomal Proteins
TLR2 protein, human
Toll-Like Receptor 2
Interleukin-4
Glyceraldehyde-3-Phosphate Dehydrogenases