Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome

J Cell Sci. 2005 Nov 1;118(Pt 21):5101-8. doi: 10.1242/jcs.02622.

Abstract

When cells enter mitosis the microtubule (MT) network undergoes a profound rearrangement, in part due to alterations in the MT nucleating and anchoring properties of the centrosome. Ninein and the ninein-like protein (Nlp) are centrosomal proteins involved in MT organisation in interphase cells. We show that the overexpression of these two proteins induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery. The ability of Nlp and ninein to perturb the cytoplasmic distribution of these organelles depends on their ability to interact with the dynein-dynactin motor complex. Our data also indicate that dynactin is required for the targeting of Nlp and ninein to the centrosome. Furthermore, phosphorylation of Nlp by the polo-like kinase 1 (Plk1) negatively regulates its association with dynactin. These findings uncover a mechanism through which Plk1 helps to coordinate changes in MT organisation with cell cycle progression, by controlling the dynein-dynactin-dependent transport of centrosomal proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / metabolism
  • Cell Cycle Proteins / physiology*
  • Cell Line
  • Centrosome / metabolism*
  • Cytoskeletal Proteins
  • Down-Regulation / physiology*
  • Dynactin Complex
  • Dyneins / metabolism
  • Dyneins / physiology*
  • GTP-Binding Proteins / metabolism
  • GTP-Binding Proteins / physiology
  • Golgi Apparatus / metabolism
  • HeLa Cells
  • Humans
  • Lysosomes / metabolism
  • Microtubule-Associated Proteins / biosynthesis
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Microtubule-Associated Proteins / physiology*
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Phosphorylation
  • Polo-Like Kinase 1
  • Protein Binding / physiology
  • Protein Kinases / metabolism
  • Protein Kinases / physiology*
  • Protein Serine-Threonine Kinases
  • Protein Structure, Tertiary / physiology
  • Protein Transport / physiology
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins / physiology*
  • Serine Endopeptidases / metabolism*
  • Transfection

Substances

  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • Dynactin Complex
  • Microtubule-Associated Proteins
  • NIN protein, human
  • NINL protein, human
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Protein Kinases
  • Protein Serine-Threonine Kinases
  • NGF-like protease
  • Serine Endopeptidases
  • GTP-Binding Proteins
  • Dyneins