Abstract
Penicillium coffeae is described as a novel endophyte isolated from a Coffea arabica L. plant in Hawaii. The species is slow growing with short, vesiculate, monoverticillate conidiophores. Phylogenetic analysis using three loci shows that P. coffeae forms a strongly supported clade with P. fellutanum, P. charlesii, P. chermesinum, P. indicum, P. phoeniceum and P. brocae. Phenotypic ally these species are quite similar but can be distinguished. The EF-1alpha gene from P. fellutanum, P. charlesii, P. chermesinum and P. indicum lack introns, P. coffeae and P. phoeniceum have a previously unknown intron at codon 20 and P. brocae and P. thiersii isolates have a single intron at codon 26. The most parsimonious interpretation of intron changes on the strongly supported phylogenetic tree requires the gain of a novel intron at position 20 and loss of intron 26 to arrive at the current distribution of introns in this gene. This is one of only a few examples of intron gain in genes.
MeSH terms
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Coffea / microbiology*
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DNA, Fungal / chemistry
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DNA, Fungal / genetics*
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Hawaii
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Introns / genetics
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Microscopy
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Microscopy, Electron, Scanning
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Molecular Sequence Data
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Penicillium / classification*
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Penicillium / genetics
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Penicillium / isolation & purification*
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Penicillium / physiology
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Peptide Elongation Factor 1 / genetics
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Phylogeny
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Sequence Analysis, DNA*
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Sequence Homology
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Spores, Fungal / cytology
Substances
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DNA, Fungal
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Peptide Elongation Factor 1
Associated data
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GENBANK/AF033399
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GENBANK/AF125936
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GENBANK/AF484391
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GENBANK/AF484392
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