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Biochem Biophys Res Commun. 2006 Mar 10;341(2):343-50. Epub 2006 Jan 19.

CK2-dependent C-terminal phosphorylation at T300 directs the nuclear transport of TSPY protein.

Author information

1
Institute of Human Genetics, Johann Wolfgang Goethe University Hospital, Frankfurt am Main, Germany.

Abstract

TSPY (testis-specific protein, Y-encoded) is a member of the greater SET/NAP family of molecules with various functions, e.g., in chromatin remodeling, regulation of gene expression, and has been implicated to play a role in the malignant development of gonadoblastoma, testicular and prostate cancer. Here we demonstrate that the C-terminus has a functional role for the nucleo-cytoplasmatic shuttling of the TSPY protein. Using various combinations of in vitro mutagenesis and enhanced green fluorescent protein reporter gene-expression experiments we were able to show that while the deletion of C-terminus leads to a decreased stability and enhanced degradation of the protein, the selective mutation of a C-terminal CK2 phosphorylation site (T300) prevents the TSPY protein from entering the nucleus. We conclude that phosphorylation of the (T300) residue is a necessary and functional prerequisite for TSPY's transport into the nucleus reminding of comparable data from a related Drosophila molecule, NAP1.

PMID:
16426576
DOI:
10.1016/j.bbrc.2005.12.190
[Indexed for MEDLINE]

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